2NVQ
RNA Polymerase II Elongation Complex in 150 mM Mg+2 with 2'dUTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Detector technology | CCD |
| Collection date | 2004-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.646, 222.760, 195.281 |
| Unit cell angles | 90.00, 101.31, 90.00 |
Refinement procedure
| Resolution | 48.450 - 2.900 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfo |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.875 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 3.100 |
| High resolution limit [Å] | 2.900 | 3.000 |
| Number of reflections | 146322 | |
| <I/σ(I)> | 8.1 | 1.4 |
| Completeness [%] | 98.9 | 94.9 |
| Redundancy | 3.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200mM NH4OAc, 150mM Mg(OAc)2, 50mM Hepes, pH 7.0, 5% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4OAc | ||
| 2 | 1 | 1 | Mg(OAc)2 | ||
| 3 | 1 | 1 | Hepes | ||
| 4 | 1 | 1 | PEG6000 | ||
| 5 | 1 | 2 | NH4OAc | ||
| 6 | 1 | 2 | Mg(OAc)2 | ||
| 7 | 1 | 2 | Hepes | ||
| 9 | 1 | 2 | PEG6000 |
