2NVN

Crystal structure of a protein with a cupin-like fold and unknown function (YP_400729.1) from Synechococcus SP. PCC 7942 (Elongatus) at 2.50 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-09
Detector	ADSC QUANTUM 315
Spacegroup name	P 43 21 2
Unit cell lengths	82.180, 82.180, 80.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.050 - 2.500
R-factor	0.209
Rwork	0.208
R-free	0.22700
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.823
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.050	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.059	0.797
Number of reflections	10011
<I/σ(I)>	13.56	1.63
Completeness [%]	99.4	97.7
Redundancy	6.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	277	2.5M NaCl, 0.1M Imidazole, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K, pH 8.00