2NVM
Crystal structure of fdxN element excision controlling factor XisI (YP_321976.1) from Anabaena Variabilis ATCC 29413 at 2.19 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000, 0.9795, 0.9792 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 122.440, 122.440, 77.076 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.152 - 2.190 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.24400 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.895 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.380 | 106.000 | 2.310 |
High resolution limit [Å] | 2.100 | 6.930 | 2.190 |
Rmerge | 0.107 | 0.014 | 1.375 |
Total number of observations | 6241 | 27353 | |
Number of reflections | 18064 | ||
<I/σ(I)> | 12.4 | 16.4 | 1.8 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 10.4 | 9.2 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 6 | 277 | 30.0% PEG-6000, 0.1M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |