2NV2
Structure of the PLP synthase complex Pdx1/2 (YaaD/E) from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.507, 259.009, 144.962 |
| Unit cell angles | 90.00, 92.13, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.120 |
| R-factor | 0.14868 |
| Rwork | 0.146 |
| R-free | 0.19789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nv1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.452 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 381798 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 98.2 | 98.4 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15-17% PEG 4000, 200mM tri-ammonium citrate pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
