2NV0
Structure of the glutaminase subunit Pdx2 (YaaE) of PLP synthase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.440, 81.606, 117.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.730 |
| R-factor | 0.18147 |
| Rwork | 0.180 |
| R-free | 0.21579 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r9g |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.706 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 45697 | |
| <I/σ(I)> | 17.3 | 2 |
| Completeness [%] | 98.4 | 99.9 |
| Redundancy | 5.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 12.4% PEG 8000, 6% ethylene glycol, 100mM cacodylic acid, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
