2NUP
Crystal Structure of the human Sec23a/24a heterodimer, complexed with the SNARE protein Sec22b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-04-24 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.200, 97.400, 129.620 |
| Unit cell angles | 90.00, 90.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| Rwork | 0.202 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1M2V and 1IFQ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.428 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
| Rmerge | 0.083 | 0.032 | 0.475 |
| Number of reflections | 55149 | ||
| <I/σ(I)> | 5 | ||
| Completeness [%] | 97.1 | 94.9 | 97.4 |
| Redundancy | 3.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 298 | 10% (w/v) PEG 4000, 0.5M sodium acetate and 50 mM Tris buffer, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
