2NUA
C123aV Mutant of E. coli Succinyl-CoA Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-23 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 97.510, 97.510, 390.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.510 - 2.950 |
| R-factor | 0.226 |
| Rwork | 0.221 |
| R-free | 0.26400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2scu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.067 | 0.421 |
| Number of reflections | 37218 | |
| <I/σ(I)> | 15.2 | 1.3 |
| Completeness [%] | 90.7 | 77.3 |
| Redundancy | 3.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 294 | Tris-HCl, ammonium sulfate, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
