2NTZ
Structure of a ParB-DNA complex reveals a double B-box interaction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 144.420, 144.420, 78.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.720 - 3.350 |
| R-factor | 0.288 |
| Rwork | 0.288 |
| R-free | 0.33600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zx4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.100 | 3.500 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 15703 | |
| <I/σ(I)> | 7.8 | 2.2 |
| Completeness [%] | 98.2 | 94 |
| Redundancy | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | MPD, MES 6.5, Magnesium chloride, sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | MES 6.5 | ||
| 3 | 1 | 1 | Magnesium chloride | ||
| 4 | 1 | 1 | sodium chloride | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | MES 6.5 | ||
| 8 | 1 | 2 | Magnesium chloride | ||
| 9 | 1 | 2 | sodium chloride |
