2NTY
Rop4-GDP-PRONE8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-03 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 63 |
| Unit cell lengths | 211.170, 211.170, 81.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.870 - 3.100 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ntx |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.926 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 | |
| High resolution limit [Å] | 3.100 | 20.000 | 3.100 |
| Rmerge | 0.160 | 0.067 | 0.439 |
| Number of reflections | 37649 | ||
| <I/σ(I)> | 16.81 | 45.2 | 4.2 |
| Completeness [%] | 99.7 | 100 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 18%(v/v) Tacsimate(Hampton Research), 4%(w/v) PEG 3350, 0.1M Tris-HCl, pH 7.3, vapor diffusion, hanging drop, temperature 293K |
