2NTW
Bacteriorhodopsin, wild type, after illumination to produce the L intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-14 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.966, 60.966, 110.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.530 |
| R-factor | 0.153 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.590 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.029 | 0.577 |
| Number of reflections | 33922 | |
| <I/σ(I)> | 42.4 | 2.1 |
| Completeness [%] | 96.9 | 86.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 295 | MO:WATER:PHOSPHATE, PH 5.6, CUBIC LIPID PHASE, TEMPERATURE 295K |
