2NTN
Crystal structure of MabA-C60V/G139A/S144L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5420 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.613, 117.285, 52.272 |
| Unit cell angles | 90.00, 121.90, 90.00 |
Refinement procedure
| Resolution | 24.000 - 2.300 |
| R-factor | 0.18055 |
| Rwork | 0.177 |
| R-free | 0.23805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uzm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.168 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.540 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.098 | 0.365 |
| Number of reflections | 17168 | |
| <I/σ(I)> | 11.1 | 3 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 316 | 22% PEG 2000, 0.35mM Mn acetate, 10% glycerol, 0.1M Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 316K |
