2NT4
Receiver domain from Myxococcus xanthus social motility protein FrzS (H92F mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-12 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.883, 37.119, 42.104 |
| Unit cell angles | 90.00, 102.49, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.020 |
| R-factor | 0.1241 |
| Rwork | 0.123 |
| R-free | 0.15390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gkg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.080 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.043 | 0.327 |
| Number of reflections | 51807 | |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 96.7 | 82.4 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 100mM Bis-Tris pH 5.5, 25% PEG 3350, 20% MPD added before freezing, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
