2NSZ
1.15 Angstrom Crystal Structure of the MA3 domain of Pdcd4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.520, 61.520, 77.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.600 - 1.150 |
| R-factor | 0.13316 |
| Rwork | 0.132 |
| R-free | 0.14959 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ion |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.451 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 1.180 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 59292 | |
| <I/σ(I)> | 28.2 | 4.54 |
| Completeness [%] | 97.0 | 99.91 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.17 M ammonium sulfate, 0.085 M sodium acetate, 25.5% (w/v) PEG 2000 MME, 15% (v/v) glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
