2NSY
CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS IN COMPLEX WITH NAD-ADENYLATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.480, 85.620, 60.120 |
| Unit cell angles | 90.00, 110.76, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| Rwork | 0.155 |
| R-free | 0.18000 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1nsy |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.033 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.300 |
| Rmerge | 0.034 |
| Total number of observations | 333971 * |
| Number of reflections | 111493 |
| Completeness [%] | 92.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.2 * | 20 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ATP | 5 (mM) | |
| 3 | 1 | drop | NAD+ | 20 (mM) | |
| 4 | 1 | reservoir | PEG400 | 20 (mM) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 6 | 1 | reservoir | 0.05 (M) |
