2NSR
Nitromethane Modified Horse Heart Myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.344, 28.740, 63.471 |
| Unit cell angles | 90.00, 106.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 1.900 |
| R-factor | 0.16298 |
| Rwork | 0.160 |
| R-free | 0.23119 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1npg |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.501 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.900 | 1.947 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 0.266 |
| Number of reflections | 9374 | |
| <I/σ(I)> | 16.3 | 4 |
| Completeness [%] | 100.0 | 66.62 |
| Redundancy | 3.53 | 2.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | Ammonium Sulfate, Tris-HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
