2NSE
BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE SUBSTRATE COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.060, 106.380, 156.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.340 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.30500 |
| Structure solution method | MAD+MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.455 |
| Data reduction software | DPS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.048 * | 0.121 * |
| Total number of observations | 131275 * | |
| Number of reflections | 40100 | |
| <I/σ(I)> | 16.9 | 3.2 |
| Completeness [%] | 96.2 | 79.5 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 15 (%) | |
| 2 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 3 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 4 | 1 | drop | SEITU | 1 (mM) | can be replaced by 5mM L-Arg |
| 5 | 1 | drop | SDS | 0.035 (mM) | |
| 6 | 1 | drop | TCEP | 5 (mM) | can be replaced by 5mM glutathione sulfonate |
