2NSB

Structures of and interactions between domains of trigger factor from Themotoga maritima

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Detector technology	CCD
Collection date	2005-02-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97917, 0.97884, 0.96864
Spacegroup name	P 21 21 2
Unit cell lengths	48.092, 86.553, 32.954
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 3.200
Rwork	0.256
R-free	0.26200
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.208
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.029	0.126
Number of reflections	2373
<I/σ(I)>	28.3
Completeness [%]	91.8	68.2
Redundancy	3	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	14% PEG 4000, 0.2M potassium chloride 0.2M, 0.1M MES, pH 6.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	6.5	293	14% PEG 4000, 0.2M potassium chloride 0.2M, 0.1M MES, pH 6.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	6.5	293	14% PEG 4000, 0.2M potassium chloride 0.2M, 0.1M MES, pH 6.5, VAPOR DIFFUSION, temperature 293K