2NSA

Structures of and interactions between domains of trigger factor from Themotoga maritim

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97900
Spacegroup name	C 2 2 21
Unit cell lengths	53.840, 74.210, 90.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
Rwork	0.227
R-free	0.25200
Structure solution method	SIRAS + native anomalous
RMSD bond length	0.004
RMSD bond angle	0.889
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.043	0.157
Number of reflections	20170
Completeness [%]	99.7	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	293	1.35M ammonium sulfate 0.05M TrisCl, pH 7.4, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	7.4	293	1.35M ammonium sulfate 0.05M TrisCl, pH 7.4, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	7.4	293	1.35M ammonium sulfate 0.05M TrisCl, pH 7.4, VAPOR DIFFUSION, temperature 293K