2NRZ
Crystal structure of the C-terminal half of UvrC bound to its catalytic divalent cation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.395, 83.853, 100.642 |
| Unit cell angles | 90.00, 99.49, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nrr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.538 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.424 |
| Number of reflections | 38877 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 98.6 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 14% PEG 8000, 0.1 M HEPES (pH 7.5), 0.01 M manganese chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
