2NRV

Crystal structure of the C-terminal half of UvrC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	35.499, 94.630, 132.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.800
R-factor	0.188
Rwork	0.185
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nrr
RMSD bond length	0.017
RMSD bond angle	1.574
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.044	0.351
Number of reflections	42358
<I/σ(I)>	15.8
Completeness [%]	99.4	99.9
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	295	15% PEG 3000, 0.1 M CHES (pH 9.5), VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	9.5	295	15% PEG 3000, 0.1 M CHES (pH 9.5), VAPOR DIFFUSION, HANGING DROP, temperature 295K