2NRR
Crystal structure of the C-terminal RNAseH endonuclase domain of UvrC
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0, 1.07249, 1.07114 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.048, 46.516, 84.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.200 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.585 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.07) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.067 | 0.417 |
| Number of reflections | 47662 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.6 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 2.4 M sodium chloride, 0.1 M sodium acetate (pH 4.6) and 0.1 M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
