2NRM
S-nitrosylated blackfin tuna myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-12 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.943, 51.932, 38.899 |
| Unit cell angles | 90.00, 103.75, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.090 |
| R-factor | 0.13429 |
| Rwork | 0.133 |
| R-free | 0.15654 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1myt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.541 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.130 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Number of reflections | 51387 | |
| <I/σ(I)> | 18.1 | 3.3 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 6.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M potassium/sodium tartrate, 0.1 M sodium citrate, and 2.0 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 193K, temperature 293K |
