2NRH
Crystal structure of conserved putative Baf family transcriptional activator from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-10-29 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.288, 73.972, 116.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.29100 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.750 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.500 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.093 | 0.933 |
| Total number of observations | 23756 | |
| Number of reflections | 23073 | |
| <I/σ(I)> | 17.6 | 1.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM MES pH 6.5, 28% PEG MME 5K, 250mM ammonium phosphate, Vapor diffusion, temperature 294K |
