2NRC
C28A Mutant of Succinyl-CoA:3-Ketoacid CoA Transferase from Pig Heart
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.300, 262.400, 60.700 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 47.900 - 2.050 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.27200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | C28S mutant |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | XFIT |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.113 | 0.257 |
| Number of reflections | 95166 | |
| <I/σ(I)> | 11.3 | 1.9 |
| Completeness [%] | 92.5 | 60.8 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 287 | PEG 2000, Tris-HCl, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
