2NR5
Crystal Structure of Protein of Unknown Function SO2669 from Shewanella oneidensis MR-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791, 0.9792 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 62.449, 69.879, 129.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.18396 |
| Rwork | 0.181 |
| R-free | 0.23066 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.449 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.103 | 0.640 |
| Number of reflections | 45319 | |
| <I/σ(I)> | 20.6 | 2 |
| Completeness [%] | 99.5 | 93.2 |
| Redundancy | 6.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 40% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
