2NR4
Crystal structure of FMN-bound protein MM1853 from Methanosarcina mazei, Pfam DUF447
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-10-29 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 63 |
| Unit cell lengths | 139.334, 139.334, 55.391 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.582 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.666 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.092 | 0.914 |
| Total number of observations | 133403 | |
| Number of reflections | 52554 | |
| <I/σ(I)> | 33.5 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.5 | 17.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Hepes pH 7.5, 8% ethylene glycol, 20% PEG 10K, VAPOR DIFFUSION, temperature 294K |
