2NPY
Crystal Structure of a junctioned hairpin ribozyme incorporating 9atom linker and 2'-deoxy 2'-amino U at A-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-31 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.290, 94.290, 131.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.420 - 2.650 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.22300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ZFR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.980 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 10389 | |
| <I/σ(I)> | 25.5 | 6 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 15.3 | 16.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG 2000 MME, lithium sulfate, cacodylate, spermidine, cobalt hexaamine, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate | ||
| 2 | 1 | 1 | spermidine | ||
| 3 | 1 | 1 | cobalt hexaamine | ||
| 4 | 1 | 1 | lithium sulfate | ||
| 5 | 1 | 1 | PEG 2000 MME | ||
| 6 | 1 | 2 | PEG 2000 MME | ||
| 7 | 1 | 2 | cacodylate | ||
| 8 | 1 | 2 | cobalt hexaamine |
