2NPX
NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.200, 134.500, 145.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.400 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.000 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.071 * |
| Number of reflections | 27682 * |
| Completeness [%] | 95.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | 20-24 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | 3 (mM) | ||
| 2 | 1 | drop | potasssium phosphate | ||
| 3 | 1 | drop | EDTA | 0.5 (mM) | |
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | potassium phosphate | ||
| 6 | 1 | reservoir | EDTA | 0.5 (mM) | |
| 7 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 8 | 1 | reservoir | ammonium sulfate | 2.05 (M) | |
| 9 | 1 | reservoir | FAD | 0.005 (mM) |
