2NPN
Crystal structure of putative cobalamin synthesis related protein (CobF) from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791, 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.104, 80.845, 76.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.17327 |
| Rwork | 0.172 |
| R-free | 0.19776 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.477 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.092 | 0.470 |
| Number of reflections | 33406 | |
| <I/σ(I)> | 14.5 | 2.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 3.5M Ammonium Chloride, 0.1 M Sodium Acetate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
