2NP5
Crystal structure of a transcriptional regulator (RHA1_ro04179) from Rhodococcus sp. Rha1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-11 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.721, 52.230, 72.853 |
| Unit cell angles | 102.31, 107.25, 89.71 |
Refinement procedure
| Resolution | 25.800 - 1.800 |
| R-factor | 0.17881 |
| Rwork | 0.177 |
| R-free | 0.21946 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.402 |
| Number of reflections | 61651 | |
| <I/σ(I)> | 24.6 | 1.9 |
| Completeness [%] | 96.7 | 94.6 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 293 | 0.2M Ca Chloride, 0.1M HEPES pH 7.4, 20% PEG3350, 0.3M NDSB195, VAPOR DIFFUSION, temperature 293K |
