2NP3
Crystal structure of TetR-family regulator (SCO0857) from Streptomyces coelicolor A3.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.431, 92.768, 93.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.590 - 2.350 |
| R-factor | 0.22462 |
| Rwork | 0.222 |
| R-free | 0.26428 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.802 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.590 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.380 | |
| Number of reflections | 16794 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 97.7 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.4 | 293 | 0.1M Hampton pH 4.4, 0.05M K Thiocyanate, 25% PEG2KMME, VAPOR DIFFUSION, temperature 293K |
