2NP2
Hbb-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.270, 88.320, 96.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.330 - 3.020 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1IHF |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.250 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Number of reflections | 8945 | |
| <I/σ(I)> | 11.33 | 1.73 |
| Completeness [%] | 85.0 | 79.9 |
| Redundancy | 5.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 100 mM TRIS (pH 7.5), 10 mM dithiothreitol (DTT), 1% Jeffamine (Hampton Research), 50 mM KCl, 20% glycerol, 15-20% PEG5000MME, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | TRIS | ||
| 10 | 1 | 2 | Jeffamine | ||
| 11 | 1 | 2 | KCl | ||
| 12 | 1 | 2 | glycerol | ||
| 13 | 1 | 2 | PEG5000MME | ||
| 14 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | dithiothreitol (DTT) | ||
| 3 | 1 | 1 | Jeffamine | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 1 | glycerol | ||
| 6 | 1 | 1 | PEG5000MME | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | TRIS | ||
| 9 | 1 | 2 | dithiothreitol (DTT) |
