2NOT
NOTECHIS II-5, NEUROTOXIC PHOSPHOLIPASE A2 FROM NOTECHIS SCUTATUS SCUTATUS
Experimental procedure
Source details | OTHER |
Temperature [K] | 298 |
Detector technology | DIFFRACTOMETER |
Collection date | 1986-02-01 |
Detector | STOE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.500, 43.500, 145.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.000 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NOTEXIN |
RMSD bond length | 0.010 |
RMSD bond angle | 23.300 * |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.130 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 4919 | |
Completeness [%] | 96.8 | 98.55 |
Redundancy | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | THE PROTEIN WAS DISSOLVED IN 50 MM TRIS-NSULFATE, 70MM NH4AC PH 8.5; TO A TOTAL CONCENTRATION OF 15MG/ML; THE PUT IN A THICK-WALLED CAPILLARY SEALED WITH A SEMI-PERMEABLE TUBING AND PLACED IN A VESSEL CONTAINING 50MM TRIS-SULFATE, 50NMM NH4AC PH 8.5.N |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-sulfate | 50 (mM) | |
2 | 1 | drop | 70 (mM) | ||
3 | 1 | drop | protein | 15 (mg/ml) | |
4 | 1 | reservoir | Tris-sulfate | 50 (mM) | |
5 | 1 | reservoir | 50 (mM) |