2NOM
Terminal uridylyl transferase 4 from Trypanosoma brucei with bound dUTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.142, 41.659, 104.286 |
Unit cell angles | 90.00, 96.46, 90.00 |
Refinement procedure
Resolution | 41.200 - 2.400 |
R-factor | 0.234 |
Rwork | 0.230 |
R-free | 0.30100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ikf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.422 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.2L) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.200 | 41.190 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.178 | 0.077 | 0.509 |
Total number of observations | 9021 | 9515 | |
Number of reflections | 26868 | ||
<I/σ(I)> | 4.9 | 10.6 | 2 |
Completeness [%] | 99.5 | 98.8 | 99.8 |
Redundancy | 3.48 | 3.18 | 3.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM sodium cacodylate pH 6.5, 200 mM magnesium chloride, 18% PEG-8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |