2NOG
SANT Domain Structure of Xenopus Remodeling Factor ISWI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2003-08-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.600, 109.500, 46.070 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.212 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ofc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 | 0.226 |
| Number of reflections | 21300 | |
| <I/σ(I)> | 20.4 | |
| Completeness [%] | 93.8 | 59.7 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 289 | 42.5% PEG 3350, 0.2 M Magnesium chloride, 0.1M HEPES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
