2NO2

Crystal structure of the DLLRKN-containing coiled-coil domain of Huntingtin-interacting protein 1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-22
Detector	NOIR-1
Wavelength(s)	0.97878, 0.97863, 0.96409
Spacegroup name	P 42 21 2
Unit cell lengths	54.200, 54.200, 152.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.429
Rwork	0.286
R-free	0.31300
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	d*TREK
Data scaling software	d*TREK (9.4LDz)
Phasing software	SOLVE (2.09)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.510	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.088	0.400
Total number of observations		9435
Number of reflections	7284
<I/σ(I)>	12.9	4.8
Completeness [%]	100.0	100
Redundancy	13.4	13.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% (v/v) PEG 3350, 0.2 M Li2SO4, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% (v/v) PEG 3350, 0.2 M Li2SO4, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% (v/v) PEG 3350, 0.2 M Li2SO4, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K