2NNN
Crystal structure of probable transcriptional regulator from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-05 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97914, 0.97928 |
| Spacegroup name | P 31 |
| Unit cell lengths | 89.373, 89.373, 217.238 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.140 - 2.400 |
| R-factor | 0.22056 |
| Rwork | 0.218 |
| R-free | 0.26222 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.279 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.568 |
| Number of reflections | 75944 | |
| <I/σ(I)> | 16.03 | 2.26 |
| Completeness [%] | 98.6 | 99.9 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 0.2M Ammonium sulfate, 0.1M Sodium acetate pH4.6, 23% PEG 4000, 0.6M PPS, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
