2NNF
Structure of the sulfur carrier protein SoxY from Chlorobium limicola f thiosulfatophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 294 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-10 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.220, 122.646, 97.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.390 |
| Rwork | 0.188 |
| R-free | 0.24800 |
| Structure solution method | Rigid Body |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.284 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.430 |
| High resolution limit [Å] | 2.390 | 6.420 | 2.390 |
| Rmerge | 0.084 | 0.037 | 0.532 |
| Number of reflections | 10173 | ||
| <I/σ(I)> | 17.5 | 3.4 | |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 4.8 | 4.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 294 | Ammonium phosphate, sodium phsophate, DTT, pH 4.0, vapor diffusion, hanging drop, temperature 294K |
