2NN5
Structure of Conserved Protein of Unknown Function EF2215 from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.227, 69.030, 86.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.520 - 1.450 |
| R-factor | 0.1535 |
| Rwork | 0.150 |
| R-free | 0.19320 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.520 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.110 | 0.582 |
| Number of reflections | 31303 | |
| <I/σ(I)> | 36.2 | 2.07 |
| Completeness [%] | 99.9 | 98.2 |
| Redundancy | 17 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.2 M magnesium chloride, 0.1 M MES buffer, 30% PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
