2NMO
Crystal structure of human galectin-3 carbohydrate-recognition domain at 1.35 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.315, 57.602, 62.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.360 - 1.350 |
| R-factor | 0.17022 |
| Rwork | 0.169 |
| R-free | 0.18441 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1a3k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.294 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.260 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.038 | 0.186 |
| Number of reflections | 28612 | |
| <I/σ(I)> | 9.7 | 4.9 |
| Completeness [%] | 97.6 | 87.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 31% PEG 6000, 100mM MgCl2, 8mM beta mercaptoethanol, 100mM Tris-HCL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
