2NMM
Crystal structure of human phosphohistidine phosphatase. Northeast Structural Genomics Consortium target HR1409
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97904, 0.97926, 0.96790 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.685, 60.088, 79.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.700 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.113 | 0.429 |
| Number of reflections | 18092 | |
| <I/σ(I)> | 14.8 | 4.6 |
| Completeness [%] | 79.4 | 82.2 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2M Ammonium Sulfate, 50 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
