2NM0

Crystal Structure of SCO1815: a Beta-Ketoacyl-Acyl Carrier Protein Reductase from Streptomyces coelicolor A3(2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-26
Detector	ADSC QUANTUM 315
Wavelength(s)	1.03155
Spacegroup name	P 31 2 1
Unit cell lengths	113.759, 113.759, 73.937
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.330 - 1.990
R-factor	0.19042
Rwork	0.189
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1io1
RMSD bond length	0.015
RMSD bond angle	1.396
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.070
High resolution limit [Å]	1.990	1.990
Rmerge	0.080	0.497
Number of reflections	37985
<I/σ(I)>	14.9	7.8
Completeness [%]	100.0	100
Redundancy	14.4	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	20% PEG MME 2000, 10 mM NiCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K