2NLY
Crystal structure of protein BH1492 from Bacillus halodurans, Pfam DUF610
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.153, 113.517, 63.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.22451 |
| Rwork | 0.222 |
| R-free | 0.27314 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.069 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.079 | 0.434 |
| Number of reflections | 10606 | |
| <I/σ(I)> | 24.6 | 4.3 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 22% PEG 8K, 100mM Sodium Acetate (pH 4.5), 200mM Lithium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
