2NLJ
Potassium Channel KcsA(M96V)-Fab complex in KCl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2002-05-11 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | I 4 |
| Unit cell lengths | 155.383, 155.383, 75.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.470 - 2.520 |
| Rwork | 0.235 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.108 | 0.089 | 0.498 |
| Number of reflections | 29644 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 96.6 | 94.4 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG400, Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
