2NLF
Human beta-defensin-1 (Mutant Leu13Glu)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.010, 46.790, 28.560 |
| Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.143 |
| Rwork | 0.140 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ijv |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.672 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.070 | 0.189 |
| Number of reflections | 13851 | |
| <I/σ(I)> | 18 | 5.8 |
| Completeness [%] | 99.0 | 97.2 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | PEG 4000, AMMONIUM SULFATE, SODIUM ACETATE, pH 4.6, vapor diffusion, temperature 293K |
