2NLD
Human beta-defensin-1 (Mutant Gln11Ala)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.840, 23.570, 40.450 |
| Unit cell angles | 90.00, 107.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.490 |
| R-factor | 0.138 |
| Rwork | 0.134 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ijv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.569 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.055 | 0.266 |
| Number of reflections | 9213 | |
| <I/σ(I)> | 26 | 4.5 |
| Completeness [%] | 92.8 | 77.2 |
| Redundancy | 5.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | PEG 4000, AMMONIUM SULFATE, SODIUM ACETATE, GLYCEROL, pH 4.6, vapor diffusion, temperature 293K |
