2MJP

STRUCTURE-BASED IDENTIFICATION OF THE BIOCHEMICAL FUNCTION OF A HYPOTHETICAL PROTEIN FROM METHANOCOCCUS JANNASCHII:MJ0226

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	44.360, 72.130, 140.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
Rwork	0.204
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	26.900 *
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.049
Number of reflections	20346
Completeness [%]	93.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	potassium phosphate	20 (mM)
3	1	drop		20 (mM)
4	1	reservoir	ADA	0.1 (M)
5	1	reservoir	PEG6000	10 (%)
6	1	reservoir	isopropanol	20 (%)