Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.700, 130.200, 81.300 |
| Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.030 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26600 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 23.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.082 | 0.173 |
| Total number of observations | 563832 * | |
| Number of reflections | 125947 | |
| <I/σ(I)> | 16 | 6 |
| Completeness [%] | 93.4 | 83.8 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8.5 | PROTEIN WAS CRYSTALLIZED USING PRECIPITATING SOLUTION OF 30% PEG 4000, 0.2M NA2MO4, 0.1M TRIS PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 30 (%) | |
| 2 | 1 | 1 | 0.2 (M) | ||
| 3 | 1 | 1 | Tris-HCl | 0.1 (M) |
