Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 49.300, 49.300, 262.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.350 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.800 | 1.800 * |
| Rmerge | 0.042 * | 0.157 * |
| Number of reflections | 28829 | 2962 * |
| Completeness [%] | 91.6 | 77.3 * |
| Redundancy | 7.5 * | 6.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 15-18 (%) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | reservoir | calcium acetate | 200 (mM) | |
| 5 | 1 | reservoir | EP-UDP-GlcNAc | 7 (mM) |
