2MAT

E.COLI METHIONINE AMINOPEPTIDASE AT 1.9 ANGSTROM RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-04-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	39.299, 67.683, 48.863
Unit cell angles	90.00, 111.24, 90.00

Refinement procedure

Resolution	35.500 - 1.900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mat
RMSD bond length	0.012
RMSD bond angle	2.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	TNT (5F PRERELEASE)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.500	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.077 *	0.251 *
Total number of observations	94123 *
Number of reflections	18444
<I/σ(I)>	22.7	6.1
Completeness [%]	100.0	100
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.1		drop consists of 1:1 mixture of well and protein solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	N-octanoyl sucrose	48.8 (mM)
3	1	reservoir	PEG4000	24-26 (%)
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir		2 (mM)